Berchtold M W, Celio M R, Heizmann C W
J Biol Chem. 1984 Apr 25;259(8):5189-96.
Parvalbumin, a high affinity Ca2+-binding protein, is known to be expressed only in muscles and brain in the rat. We have investigated its distribution and characteristics in other rat tissues by several biochemical and immunohistochemical methods. Evidence for the presence of parvalbumin in teeth, bone, skin, prostate, seminal vesicles, testes, and ovary is given by two-dimensional polyacrylamide gel electrophoresis, immunoblotting ("Western technique") of one-dimensional gels, and its concentration measured by reverse phase high performance liquid chromatography. The distribution within several parvalbumin-positive organs was monitored by the immunohistochemical peroxidase-antiperoxidase method. In teeth, only ameloblasts reacted with anti-rat parvalbumin serum and in bone the calcified extracellular cartilage was the target of the immunoreaction. The panniculus carnosus was the exclusive site of parvalbumin in the skin. Besides the already known parvalbumin distribution in the brain, parvalbumin is also expressed in distinct cell types of the peripheral nervous system. Leydig cells were found to be the only parvalbumin location in testes. These observations lead us to conclude that parvalbumin in contrast to the multifunctional and constitutive calmodulin must function in Ca2+-dependent processes related to specific cell types.
小白蛋白是一种高亲和力的钙结合蛋白，已知仅在大鼠的肌肉和大脑中表达。我们通过多种生化和免疫组织化学方法研究了它在大鼠其他组织中的分布和特性。二维聚丙烯酰胺凝胶电泳、一维凝胶的免疫印迹法（“Western技术”）以及反相高效液相色谱法测定其浓度，均证实了牙齿、骨骼、皮肤、前列腺、精囊、睾丸和卵巢中存在小白蛋白。通过免疫组织化学过氧化物酶-抗过氧化物酶法监测了几种小白蛋白阳性器官内的分布情况。在牙齿中，只有成釉细胞与抗大鼠小白蛋白血清发生反应，在骨骼中，钙化的细胞外软骨是免疫反应的靶点。皮肤中的肉膜是小白蛋白的唯一存在部位。除了已知的大脑中存在小白蛋白分布外，小白蛋白还在外周神经系统的不同细胞类型中表达。发现睾丸间质细胞是睾丸中唯一存在小白蛋白的部位。这些观察结果使我们得出结论，与多功能的组成型钙调蛋白不同，小白蛋白必须在与特定细胞类型相关的钙依赖过程中发挥作用。
