Sanna P P, Celio M R, Bloom F E, Rende M
Department of Neuropharmacology, Scripps Research Institute, La Jolla, CA 92037.
Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):3048-52. doi: 10.1073/pnas.90.7.3048.
A subpopulation of calbindin-immunoreactive neurons in lamina VII of the spinal cord has been identified by its location as Renshaw cells, the anatomical substrate for recurrent inhibition. The expression of calbindin (28 kDa) in these calbindin-containing rat ventral horn interneurons was studied with immunocytochemistry after sciatic nerve injuries. One week after axotomy calbindin immunoreactivity was strongly reduced on the lesioned side between levels L4 and L6, while calbindin-containing neurons and fibers were still numerous contralaterally and cranially to the lesioned levels. With the progression of regeneration, calbindin-immunoreactive neurons reappeared, reaching a normal distribution 6-8 weeks after the crush. Similar changes could be mimicked by the intramuscular administration of botulinum toxin. These results suggest that calbindin expression in putative Renshaw cells of the spinal cord might be functionally responsive and that maintenance of calbindin expression may depend on the integrity of motoneurons and neuromuscular transmission.
脊髓第VII层中一群钙结合蛋白免疫反应阳性神经元，根据其位置已被确定为闰绍细胞，即反复抑制的解剖学基础。在坐骨神经损伤后，用免疫细胞化学方法研究了这些含钙结合蛋白的大鼠腹角中间神经元中钙结合蛋白（28 kDa）的表达。轴突切断术后一周，在L4至L6节段的损伤侧，钙结合蛋白免疫反应性显著降低，而在损伤节段的对侧和头侧，含钙结合蛋白的神经元和纤维仍然很多。随着再生的进展，钙结合蛋白免疫反应阳性神经元重新出现，挤压后6 - 8周达到正常分布。肌肉注射肉毒杆菌毒素可模拟类似变化。这些结果表明，脊髓中假定的闰绍细胞中的钙结合蛋白表达可能具有功能反应性，并且钙结合蛋白表达的维持可能依赖于运动神经元和神经肌肉传递的完整性。
