Hagag N, McPherson R A, Birnbaum E R, Darnall D W
J Biol Chem. 1984 May 10;259(9):5411-3.
Reaction of human serum albumin with p-nitrophenylanthranilate results in transesterification of the anthraniloyl group to tyrosine 411. Titration of anthraniloyl-Tyr-411-albumin with long chain or short chain fatty acids produces marked changes in the absorption and fluorescence spectra of the anthraniloyl moiety as fatty acids bind in the channel near it. It appears that the anthraniloyl group is a very sensitive probe that can follow binding of small molecules at the 3-AB subdomain of human serum albumin.
人血清白蛋白与对硝基苯基邻氨基苯甲酸反应会导致邻氨基苯甲酰基转移至酪氨酸411。用长链或短链脂肪酸滴定邻氨基苯甲酰基-酪氨酸-411-白蛋白时，随着脂肪酸在其附近的通道中结合，邻氨基苯甲酰基部分的吸收光谱和荧光光谱会发生显著变化。看来邻氨基苯甲酰基是一种非常敏感的探针，能够追踪人血清白蛋白3-AB亚结构域处小分子的结合情况。
