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短重复长度染色质中的高阶结构。

Higher order structure in a short repeat length chromatin.

作者信息

Allan J, Rau D C, Harborne N, Gould H

出版信息

J Cell Biol. 1984 Apr;98(4):1320-7. doi: 10.1083/jcb.98.4.1320.

Abstract

Polynucleosomes from calf brain cortical neurone nuclei have an average repeat length of less than 168 base pairs. The ability of this material to adopt higher order structure has been assessed by various physical techniques. Although containing on average less DNA per nucleosome than is required to form a chromatosome, this short repeat length chromatin folded in an H1 dependent manner to a structure with properties similar to those observed for longer repeat length chromatins such as that of chicken erythrocyte (McGhee, J.D., D.C. Rau, E. Charney, and G. Felsenfeld, 1980, Cell, 22:87-96). These observations are discussed in the context of H1 location in the higher order chromatin fiber.

摘要

来自小牛脑皮质神经元细胞核的多核小体平均重复长度小于168个碱基对。已通过各种物理技术评估了这种物质形成高级结构的能力。尽管每个核小体平均含有的DNA比形成染色质小体所需的DNA少,但这种短重复长度的染色质以H1依赖的方式折叠成一种结构,其性质与在较长重复长度的染色质(如鸡红细胞染色质)中观察到的性质相似(McGhee, J.D., D.C. Rau, E. Charney, and G. Felsenfeld, 1980, Cell, 22:87 - 96)。在高级染色质纤维中H1的定位背景下讨论了这些观察结果。

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