Chromatographic profiling of urinary volatile and organic acid metabolites of normal and diabetic C57BL/Ks mice.

Capillary gas chromatography and gas chromatography-mass spectrometry were used to examine the urinary volatile and organic acid metabolites in normal and diabetic C57BL/Ks male mice. Quantitative differences in the excretion of these metabolites were assessed in the animals from 5 to 24 weeks of age. Statistically significant differences were examined with respect to known metabolic abnormalities in the diabetic animals and to the possible toxic effects of elevated levels of certain metabolites. A number of aldehyde metabolites and aromatic acids, as well as most other organic acids, were found at consistently higher levels in diabetic urine. Several ketone metabolites, linalool, and 2-sec.-butylthiazoline were found at consistently low levels throughout the study.