Gaffney M H, Cooke M, Simpson R
J Chromatogr. 1984 Mar 9;306:303-13. doi: 10.1016/s0378-4347(00)80892-0.
A high-performance liquid chromatographic method is described which is suitable for the determination of the common antiseptic chlorhexidine in urine. The method employs Sep-Pak cartridges to remove chlorhexidine from the urine matrix. Chromatographic separation was achieved on a C18 reversed-phase column using a mobile phase of methanol-20 mM sodium acetate solution (60:40) adjusted to pH 5 with glacial acetic acid. An ion-pair agent (pentadecafluorooctanoic acid) was used at a concentration of 100 micrograms ml-1 X 3-Bromobenzophenone was used as chromatographic standard (kappa' = 4.0). 4-Bromobenzophenone (kappa' = 3.9) or dibenzal hydrazine (kappa' = 4.4) may also be used. A series of urine samples was analysed and no interferences were observed. The method is simple and rapid with a total analysis time of ca. 30 min.
本文描述了一种高效液相色谱法，该方法适用于测定尿液中常见的防腐剂洗必泰。该方法采用Sep-Pak柱从尿液基质中去除洗必泰。在C18反相柱上进行色谱分离，流动相为甲醇-20 mM醋酸钠溶液（60:40），用冰醋酸调节至pH 5。使用浓度为100微克/毫升的离子对试剂（十五氟辛酸）。以3-溴二苯甲酮为色谱标准品（容量因子κ' = 4.0）。也可使用4-溴二苯甲酮（κ' = 3.9）或二苄叉肼（κ' = 4.4）。分析了一系列尿液样本，未观察到干扰。该方法简单快速，总分析时间约为30分钟。
