Brougham L R, Cheng H Y, Pittman K A
J Chromatogr. 1986 Dec 19;383(2):365-73. doi: 10.1016/s0378-4347(00)83482-9.
A high-performance liquid chromatographic method for the analysis of chlorhexidine in human serum and urine was developed. Chlorhexidine and the internal standard, chlorpheniramine, were extracted into chloroform, containing 5% 2-propanol, and back-extracted into dilute sulfuric acid. Chromatographic separation was achieved on a C18 column equilibrated with methanol-water (70:30, v/v), containing 0.005 M sodium heptane-sulfonate. The sensitivity of the assay was 20 ng/ml of biological matrix, using 0.5-ml samples. The application of this method to monitor chlorhexidine levels in burn patients treated topically with a chlorhexidine-containing burn cream was demonstrated.
建立了一种用于分析人血清和尿液中洗必泰的高效液相色谱法。将洗必泰和内标物氯苯那敏萃取到含5%异丙醇的氯仿中，然后反萃取到稀硫酸中。在以甲醇 - 水（70:30，v/v）平衡且含有0.005 M庚烷磺酸钠的C18柱上实现色谱分离。使用0.5 ml样品时，该测定方法的灵敏度为生物基质中20 ng/ml。证明了该方法在监测局部使用含洗必泰烧伤膏治疗的烧伤患者体内洗必泰水平方面的应用。
