Redman L W, Tustanoff E R
J Immunoassay. 1984;5(1-2):29-57. doi: 10.1080/01971528408062997.
Antigen-antibody kinetics were studied using a hapten which was iodinated by two unique procedures. Using bradykinin, a vasopressor hormone as a model peptide, radioactive iodination (125I) of its 8-tyrosyl analogue was carried out both enzymatically and chemically using modified procedures. Two distinct chemical species were obtained which were characterized on a chromatographic, chemical as well as charge basis as a mono-iodinated form of [Tyr8]-bradykinin using the lactoperoxidase procedure and a di-iodinated entity using chloramine-T technique. The addition of a second iodine atom to the antigen lowers its immunoreactivity for its antibody and thus alters the kinetics of this reaction. Further experiments on the stability (temperature, time of storage, and chemical environment) of these iodinated peptides are described.
使用通过两种独特方法碘化的半抗原研究了抗原-抗体动力学。以血管加压素激素缓激肽作为模型肽，使用改良方法通过酶促和化学方法对其8-酪氨酰类似物进行放射性碘化（125I）。得到了两种不同的化学物质，通过色谱、化学以及电荷分析，使用乳过氧化物酶法鉴定为[酪氨酸8]-缓激肽的单碘化形式，使用氯胺-T技术鉴定为双碘化实体。向抗原中添加第二个碘原子会降低其对抗体的免疫反应性，从而改变该反应的动力学。本文还描述了对这些碘化肽的稳定性（温度、储存时间和化学环境）的进一步实验。
