Harper J M, Parsonage M T, Pelham H R, Darby G
J Virol. 1978 Jun;26(3):646-59. doi: 10.1128/JVI.26.3.646-659.1978.
The heat inactivation characteristics of several vaccinia virus particle-associated functions known to be involved in the transcription of the genome were examined. All functions were more resistant to heat than infectivity. Noninfectious particles were generated which exhibited significant levels of activity of all enzymes examined, and their properties were investigaed both in vitro and in vivo. RNA was synthesized in vitro by such particles, although transport of the RNA into the surrounding medium was defective. This RNA was larger than that made in normal particles but it was polyadenylated and functioned in vitro as a message coding for normal early proteins. The sequences transcribed were similar to those transcribed in normal particles, and we suggest that the production of abnormally large RNA is due to a defect in transcriptional termination. We could not detect any virus-specific protein or RNA synthesis in cells exposed to these inactivated particles and conclude that the loss of infectivity caused by heating is due to a general decline in the activities of a number of particle functions.
对几种已知参与基因组转录的痘苗病毒颗粒相关功能的热灭活特性进行了研究。所有功能对热的抗性都比感染性更强。产生了无感染性的颗粒，其表现出所检测的所有酶的显著活性水平，并在体外和体内对其特性进行了研究。此类颗粒在体外合成了RNA，尽管RNA向周围培养基的转运存在缺陷。这种RNA比正常颗粒中产生的RNA更大，但它是聚腺苷酸化的，并且在体外作为编码正常早期蛋白质的信使发挥作用。转录的序列与正常颗粒中转录的序列相似，我们认为异常大的RNA的产生是由于转录终止缺陷所致。在暴露于这些灭活颗粒的细胞中，我们检测不到任何病毒特异性蛋白质或RNA合成，并得出结论，加热导致的感染性丧失是由于许多颗粒功能的活性普遍下降所致。
