Gold Marielle C, Robinson Tammie L, Cook Matthew S, Byrd Laura K, Ehlinger Heather D, Lewinsohn David M, Lewinsohn Deborah A
Department of Pulmonary and Critical Care Medicine, Oregon Health & Science University, Portland, Oregon, USA.
PLoS One. 2007 Sep 26;2(9):e957. doi: 10.1371/journal.pone.0000957.
Neonates are clearly more susceptible to severe disease following infection with a variety of pathogens than are adults. However, the causes for this are unclear and are often attributed to immunological immaturity. While several aspects of immunity differ between adults and neonates, the capacity of dendritic cells in neonates to process and present antigen to CD8+ T cells remains to be addressed. We used human CD8+ T cell clones to compare the ability of neonatal and adult monocyte-derived dendritic cells to present or process and present antigen using the MHC class I pathway. Specifically, we assessed the ability of dendritic cells to present antigenic peptide, present an HLA-E-restricted antigen, process and present an MHC class I-restricted antigen through the classical MHC class I pathway, and cross present cell-associated antigen via MHC class I. We found no defect in neonatal dendritic cells to perform any of these processing and presentation functions and conclude that the MHC class I antigen processing and presentation pathway is functional in neonatal dendritic cells and hence may not account for the diminished control of pathogens.
与成年人相比，新生儿显然更容易在感染各种病原体后患上严重疾病。然而，其原因尚不清楚，通常归因于免疫不成熟。虽然成年人和新生儿在免疫的几个方面存在差异，但新生儿树突状细胞处理抗原并将其呈递给CD8 + T细胞的能力仍有待研究。我们使用人类CD8 + T细胞克隆，比较新生儿和成人单核细胞衍生的树突状细胞使用MHC I类途径呈递或处理并呈递抗原的能力。具体而言，我们评估了树突状细胞呈递抗原肽、呈递HLA-E限制性抗原、通过经典MHC I类途径处理并呈递MHC I类限制性抗原以及通过MHC I类交叉呈递细胞相关抗原的能力。我们发现新生儿树突状细胞在执行这些处理和呈递功能方面没有缺陷，并得出结论，MHC I类抗原处理和呈递途径在新生儿树突状细胞中起作用，因此可能不是病原体控制减弱的原因。
