Guardini M A, Schoenberg M P, Naso R B, Martin B A, Gutterman J U, Guevara J
J Interferon Res. 1984;4(1):67-79. doi: 10.1089/jir.1984.4.67.
Two-dimensional gel electrophoresis, using either silver staining or pulse labeling with 35S-L-methionine and autoradiography, was employed to determine changes in the synthesis of proteins that may be involved in the antiproliferative effects of recombinant alpha interferon (IFNrA) on Burkitt's lymphoma Daudi cells. IFNrA initiated and/or augmented the synthesis of at least 13 proteins that were distinct in molecular weights and isoelectric properties. Synthesis of several of these IFN-enhanced proteins was inhibited by actinomycin-D, an inhibitor of mRNA synthesis. Although IFN-induced antiproliferative effects were observed at 48 h, an increase in the synthesis of several proteins was observed as early as 3 h. The levels of these IFN-enhanced proteins in treated cells continued to increase through 24 h. At least two proteins of approximately 17 kD were observed to be synthesized in IFN-treated cells but not in control cells. Neither inhibition of synthesis of any particular protein nor post-synthetic modification of proteins in response to IFNrA was observed with these methods. The results of this study are compared and contrasted to those of several other laboratories.
采用二维凝胶电泳法，通过银染或用³⁵S-L-甲硫氨酸脉冲标记及放射自显影，来确定可能参与重组α干扰素（IFNrA）对伯基特淋巴瘤Daudi细胞抗增殖作用的蛋白质合成变化。IFNrA启动和/或增强了至少13种蛋白质的合成，这些蛋白质在分子量和等电性质上各不相同。其中几种IFN增强的蛋白质的合成被放线菌素-D（一种mRNA合成抑制剂）所抑制。虽然在48小时时观察到IFN诱导的抗增殖作用，但早在3小时就观察到几种蛋白质的合成增加。处理细胞中这些IFN增强的蛋白质水平在24小时内持续增加。在IFN处理的细胞中观察到至少两种约17kD的蛋白质被合成，而在对照细胞中未观察到。使用这些方法未观察到对任何特定蛋白质合成的抑制或蛋白质对IFNrA的合成后修饰。本研究的结果与其他几个实验室的结果进行了比较和对比。
