Grinblat J, Klein A
J Endocrinol. 1984 May;101(2):149-53. doi: 10.1677/joe.0.1010149.
A study was carried out to determine the effect on lymphocytic cortisol metabolism (LCM) of plasma from 62 patients with diffuse thyrotoxic goitre (DTG), 14 patients with toxic nodular goitre (TNG) and ten hypothyroid patients. Plasma of 33 healthy donors served as controls. A known concentration of human lymphocytes was incubated with cortisol in media containing 50% phosphate-buffered saline (PBS) and 50% of one of the following additions: (1) PBS, (2) homologous plasma (HP), (3) heterologous plasma, (4) plasma from DTG patients, (5) plasma from TNG patients, (6) plasma from hypothyroid patients, (7) PBS and HP to which L-thyroxine (T4) and triiodothyronine (T3) had been added separately and as a mixture up to a concentration ten times the normal, (8) boiled HP and (9) boiled DTG plasma. Plasma from hypothyroid patients gave an LCM-enhancing effect (LCMEE) similar to that of HP. The plasma of DTG and TNG patients had a markedly lesser effect on LCM than did HP. The T4 and T3 had no additional effect when added to PBS or HP. Boiling of HP and DTG plasma resulted in a similar decrease in LCMEE. The findings of this study raise the possibility of the existence of a factor inhibiting LCMEE in the plasma of thyrotoxic patients.
开展了一项研究，以确定62例弥漫性毒性甲状腺肿（DTG）患者、14例毒性结节性甲状腺肿（TNG）患者和10例甲状腺功能减退患者的血浆对淋巴细胞皮质醇代谢（LCM）的影响。33名健康供体的血浆作为对照。将已知浓度的人淋巴细胞与皮质醇在含有50%磷酸盐缓冲盐水（PBS）和50%以下添加物之一的培养基中孵育：（1）PBS，（2）同源血浆（HP），（3）异源血浆，（4）DTG患者的血浆，（5）TNG患者的血浆，（6）甲状腺功能减退患者的血浆，（7）分别添加L-甲状腺素（T4）和三碘甲状腺原氨酸（T3）以及以浓度为正常浓度10倍的混合物添加到PBS和HP中的添加物，（8）煮沸的HP，（9）煮沸的DTG血浆。甲状腺功能减退患者的血浆产生的淋巴细胞皮质醇代谢增强效应（LCMEE）与HP相似。DTG和TNG患者的血浆对LCM的影响明显小于HP。将T4和T3添加到PBS或HP中没有额外影响。煮沸HP和DTG血浆导致LCMEE出现类似下降。本研究结果增加了甲状腺毒症患者血浆中存在抑制LCMEE因子的可能性。
