Bolton V N, Oades P J, Johnson M H
J Embryol Exp Morphol. 1984 Feb;79:139-63.
The 2-cell stage of mouse embryogenesis is characterized by two phases of alpha-amanitin-sensitive polypeptide synthetic activity, which appear to mark the first major expression of the embryonic genome, as assessed by examination of in vitro translates of mRNA. Using populations of embryos synchronized to the first cleavage division, we have established that DNA replication takes place over the period 1 to 5.5 h after the first cleavage division; the two bursts of putative transcription take place before and immediately after DNA replication, and the translation products are detectable in each case within 3-4 h. In addition, we have shown that suppression of cytokinesis and the second round of DNA replication does not affect synthesis of the alpha-amanitin-sensitive polypeptides, and that neither DNA replication nor the loss of maternal mRNA that take place during the 2-cell stage are dependent upon synthesis of the alpha-amanitin-sensitive polypeptides.
小鼠胚胎发育的2细胞期的特征是存在两个对α-鹅膏蕈碱敏感的多肽合成活性阶段，通过对mRNA的体外翻译产物进行检测评估，这两个阶段似乎标志着胚胎基因组的首次主要表达。利用同步到第一次卵裂的胚胎群体，我们确定DNA复制发生在第一次卵裂后的1至5.5小时；两次假定的转录爆发分别发生在DNA复制之前和之后，并且在每种情况下，翻译产物在3 - 4小时内均可检测到。此外，我们还表明，抑制胞质分裂和第二轮DNA复制并不影响α-鹅膏蕈碱敏感多肽的合成，并且在2细胞期发生的DNA复制和母体mRNA的丢失均不依赖于α-鹅膏蕈碱敏感多肽的合成。
