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使用格瑞纳G 300进行尿素的酶法测定。

The enzymatic determination of urea using the Greiner G 300.

作者信息

Weber J A

出版信息

J Clin Chem Clin Biochem. 1984 Feb;22(2):195-7.

PMID:6716059
Abstract

A diagnostic kit for the enzymatic determination of urea was evaluated. The with-in-run had a coefficient of variation of 1.9-2.4%. The day-to-day run precision was found to have a coefficient of variation of 2.1 - 3.5%. The mean analytical recovery was 97.5%. A correlation study was done with the Technicon Mark II method. Good linearity was found up to 30 mmol/l; after the addition of NADH linearity was extended up to 60 mmol/l. The working reagent was stable for two days.

摘要

对一种用于酶法测定尿素的诊断试剂盒进行了评估。批内变异系数为1.9 - 2.4%。发现日间测定精密度的变异系数为2.1 - 3.5%。平均分析回收率为97.5%。与Technicon Mark II方法进行了相关性研究。发现高达30 mmol/l时具有良好的线性;加入NADH后线性范围扩展至60 mmol/l。工作试剂可稳定保存两天。

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