Orsonneau J L, Meflah K, Cornu G, Bernard S
Ann Biol Clin (Paris). 1980;38(2):139-42.
An automatic continuous flow method is described for assay of plasmatic and urinary urea after hydrolysis by urease followed by NH4 assay with glutamic deshydrogenase in the same working solution. Precision and accuracy, comparison with chemical diacetylmonoxim method are studied. The interaction of endogenous NH4, the cost of the method are calculated.
本文描述了一种自动连续流动法,用于在脲酶水解后,在同一工作溶液中用谷氨酸脱氢酶测定血浆和尿液中的尿素,随后测定铵。研究了该方法的精密度和准确度,并与化学二乙酰一肟法进行了比较。计算了内源性铵的相互作用以及该方法的成本。