Fulop G M, McMillan D B
J Morphol. 1984 Feb;179(2):175-95. doi: 10.1002/jmor.1051790205.
A histological investigation of the filtering function of the spleen of the sunfish Lepomis spp. was conducted by light, scanning, and transmission electron microscopy. The parenchyma of the organ is predominantly red pulp, a system of splenic cords and sinuses. The white pulp consists of loose lymphoid tissue which forms a cuff around the pulp arteries. Filtering of particulate matter from the blood occurs in the red pulp by phagocytes of the pulp cords and ellipsoids (periarterial macrophage sheaths). The ellipsoids are pale-staining cuffs of macrophages and reticular cells in a framework of reticular fibres surrounding the arterial capillaries. Destruction of effete blood cells (especially erythrocytes) is confined to the pigment nodules; particulate matter is not taken up by the nodules. These yellow-brown bodies are dispersed throughout the red pulp and are bounded by a reticular capsule. They contain masses of phagocytes and have the appearance of a morula. They are associated with blood vessels and are surrounded by sinusoids. Prussian Blue stain shows the presence of haemosiderin within their phagocytes. The phagocytes of the pigment nodules are filled with inclusions such as residual bodies, siderosomes, and fragments of erythrocytes. The early filtering of particulate matter by the phagocytes of the pulp cords and ellipsoids may allow for a more efficient phagocytosis of erythrocytes by the pigment nodules, followed by storage and reutilization of iron-containing compounds uncontaminated by other phagocytosed material.
利用光学显微镜、扫描电子显微镜和透射电子显微镜对太阳鱼属（Lepomis spp.）脾脏的滤过功能进行了组织学研究。该器官的实质主要是红髓，即脾索和脾窦系统。白髓由疏松的淋巴组织组成，围绕着髓动脉形成一个套层。血液中的颗粒物在红髓中由脾索和椭球体（动脉周围巨噬细胞鞘）的吞噬细胞进行滤过。椭球体是巨噬细胞和网状细胞的淡染套层，位于围绕动脉毛细血管的网状纤维框架内。衰老血细胞（尤其是红细胞）的破坏局限于色素结节；颗粒物不会被结节摄取。这些黄棕色小体分散在整个红髓中，被网状被膜所包绕。它们含有大量吞噬细胞，外观像桑葚胚。它们与血管相关，周围有血窦。普鲁士蓝染色显示其吞噬细胞内存在含铁血黄素。色素结节的吞噬细胞充满了诸如残余体、含铁小体和红细胞碎片等内含物。脾索和椭球体的吞噬细胞对颗粒物的早期滤过可能使色素结节对红细胞进行更有效的吞噬作用，随后储存和再利用未被其他吞噬物质污染的含铁化合物。
