在用于选择次黄嘌呤鸟嘌呤磷酸核糖转移酶（HGPRT）回复突变体的含有氨基蝶呤或重氮丝氨酸的培养基中获得具有不同表型的V79细胞克隆。

Recovery of V79 cell clones with different phenotypes in aminopterin- or azaserine-containing media used for the selection of HGPRT revertants.

作者信息

Randazzo R, Bonatti S, Luccarini G, Abbondandolo A

出版信息

Mutat Res. 1984 May;126(3):273-7. doi: 10.1016/0027-5107(84)90007-1.

Abstract

Three 6-thioguanine (6TG)-resistant mutants were mutagen-treated and selected for clones capable of growing in 2 selective media: HAT medium, containing aminopterin (AP) and HAS medium, containing L-azaserine (AS). Both 6TG-sensitive, wild-type clones and 6TG-resistant mutants were found among colonies growing in HAT medium, while only 6TG-sensitive clones grew in HAS medium. Time for expression was required by 6TG-resistant but not by 6TG-sensitive clones, that were fully expressed immediately after treatment. All HAT-resistant, 6TG-resistant clones which were analyzed proved to be resistant to AP. These data were interpreted as follows: in HAT medium, both HGPRT+ revertants and double mutants (HGPRT-, AP-resistant) were selected, while only HGPRT+ revertants were selected in HAS medium. Not all 6TG-resistant mutants were able to produce both classes of HAT-resistant clones.

摘要

对三个6-硫鸟嘌呤（6TG）抗性突变体进行诱变处理，并筛选出能够在两种选择培养基中生长的克隆：含有氨基蝶呤（AP）的HAT培养基和含有L-氮杂丝氨酸（AS）的HAS培养基。在HAT培养基中生长的菌落中发现了6TG敏感的野生型克隆和6TG抗性突变体，而在HAS培养基中只有6TG敏感的克隆生长。6TG抗性克隆需要表达时间，而6TG敏感克隆在处理后立即完全表达。所有经分析的HAT抗性、6TG抗性克隆均被证明对AP具有抗性。这些数据的解释如下：在HAT培养基中，选择了HGPRT+回复突变体和双突变体（HGPRT-，AP抗性），而在HAS培养基中只选择了HGPRT+回复突变体。并非所有6TG抗性突变体都能产生两类HAT抗性克隆。