Stone-Wolff D S, Klein C B, Rossman T G
Environ Mutagen. 1985;7(3):281-91. doi: 10.1002/em.2860070305.
In order to determine the mutagenic specificity of mutagenic and carcinogenic agents in mammalian cells, a reversion system capable of distinguishing between frameshift mutations and various kinds of base pair substitutions would be useful. We report here a method for the isolation and characterization of HGPRT- Chinese hamster V79 cell mutants that might form the basis for such a system. Two mutants of different specificity have been partially characterized. DEW-1, isolated following N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) treatment, is revertible by the base pair substitution mutagens MNNG and ethyl methanesulfonate (EMS), but not by frameshift mutagens. DSW-3, isolated following ICR-191 treatment, is specifically reverted by frameshift mutagens, but not by EMS or MNNG. With the further characterization of these and other mutants, it should be feasible to determine not only whether an agent is mutagenic in V79 cells, but also to determine the type(s) of mutation(s) it produces.
为了确定诱变剂和致癌剂在哺乳动物细胞中的诱变特异性，一个能够区分移码突变和各种碱基对替换的回复系统将很有用。我们在此报告一种分离和鉴定HGPRT-中国仓鼠V79细胞突变体的方法，该方法可能构成这样一个系统的基础。已对两个不同特异性的突变体进行了部分表征。DEW-1是在N-甲基-N'-硝基-N-亚硝基胍（MNNG）处理后分离得到的，可被碱基对替换诱变剂MNNG和甲磺酸乙酯（EMS）回复，但不能被移码诱变剂回复。DSW-3是在ICR-191处理后分离得到的，可被移码诱变剂特异性回复，但不能被EMS或MNNG回复。随着对这些及其他突变体的进一步表征，不仅确定一种试剂在V79细胞中是否具有诱变性，而且确定它产生的突变类型应该是可行的。
