通过辐射失活法对昆虫胆碱能受体的分子量估计

Molecular weight estimates of insect cholinergic receptors by radiation inactivation.

作者信息

Lummis S C, Sattelle D B, Ellory J C

出版信息

Neurosci Lett. 1984 Jan 27;44(1):7-12. doi: 10.1016/0304-3940(84)90212-x.

Abstract

Molecular weight (MW) estimates for sites to which the radiolabelled cholinergic receptor probes alpha-bungarotoxin and N-methylscopolamine bind in CNS extracts of the cockroach Periplaneta americana have been made by radiation inactivation analysis. The MW of 77,600 determined for [3H]N-methylscopolamine binding sites agrees well with published values for vertebrate muscarinic acetylcholine receptor sites. In contrast, N-[propionyl-3H]propionylated alpha-bungarotoxin binds to a separate membrane component of lower MW (108,000) than previously reported values for vertebrate and insect nicotinic acetylcholine receptors. This appears to represent one or more subunits of the receptor complex, containing the recognition site.

摘要

通过辐射失活分析，已对放射性标记的胆碱能受体探针α-银环蛇毒素和N-甲基东莨菪碱在美洲大蠊中枢神经系统提取物中结合位点的分子量（MW）进行了估算。为[³H]N-甲基东莨菪碱结合位点测定的77,600分子量与已发表的脊椎动物毒蕈碱型乙酰胆碱受体位点的值非常吻合。相比之下，N-[丙酰基-³H]丙酰化的α-银环蛇毒素与分子量较低（108,000）的单独膜成分结合，该值低于先前报道的脊椎动物和昆虫烟碱型乙酰胆碱受体的值。这似乎代表了受体复合物的一个或多个亚基，其中包含识别位点。