Sapunova E G
Probl Endokrinol (Mosk). 1984 Mar-Apr;30(2):85-9.
The system has been developed for radioimmunologic determination of the blood prolactin content in domestic animals--pig, sheep and bull, using common antiserum for the pig prolactin. This antiserum has a high antibody titer and can bind with large affinity not only the pig but also sheep and bull prolactins. Incubation system contains a final volume of 300 microliter of antiserum (1:192 000), 125I5-labelled pig hormone (30 000 imp/min) and standard or dofining hormone (0.5 to 100 micrograms/ml). To separate the free- and antibody-bound hormone fractions the double antibody method was used.
该系统已开发用于通过放射免疫法测定家畜（猪、绵羊和公牛）血液中的催乳素含量，使用的是针对猪催乳素的普通抗血清。这种抗血清具有高抗体效价，不仅能与猪催乳素，还能与绵羊和公牛催乳素以高亲和力结合。温育系统的最终体积为300微升抗血清（1:192 000）、125I标记的猪激素（30 000脉冲/分钟）和标准或待测激素（0.5至100微克/毫升）。采用双抗体法分离游离激素和抗体结合激素部分。
