猪催乳素的放射免疫测定：泌乳、哺乳和断奶期间以及注射促甲状腺激素释放激素后的血浆水平。

Radioimmunoassay for porcine prolactin: plasma levels during lactation, suckling and weaning and after TRH administration.

作者信息

van Landehem A A, van de Wiel D F

出版信息

Acta Endocrinol (Copenh). 1978 Aug;88(4):653-67. doi: 10.1530/acta.0.0880653.

DOI:10.1530/acta.0.0880653

Abstract

A sensitive heterologous radioimmunoassay for porcine prolactin (pPRL) has been developed. Anti-ovine prolactin antibody was raised in rabbits which allowed a final dilution of 1:500 000. The separation of free and antibody bound [125I]pPRL is based on the double antibody solid phase system. The assay is specific for pPRL. There is no cross-reaction with pLH, pFSH and pTSH; little cross-reaction (1.35%) was found with pGH. The smallest detectable amount was 0.08 ng per tube. During lactation high plasma levels are found with great fluctuations. After weaning the plasma PRL levels fall to basal within a few hours. After thyrotrophin-releasing hormone (TRH) administration plasma concentrations increase within a few minutes.

摘要

已开发出一种用于猪催乳素（pPRL）的灵敏异源放射免疫测定法。在兔中制备了抗羊催乳素抗体，最终稀释度可达1:500 000。游离的和与抗体结合的[125I]pPRL的分离基于双抗体固相系统。该测定法对pPRL具有特异性。与pLH、pFSH和pTSH无交叉反应；与pGH有轻微交叉反应（1.35%）。每管最小可检测量为0.08 ng。在哺乳期，血浆水平较高且波动较大。断奶后，血浆PRL水平在数小时内降至基础水平。给予促甲状腺激素释放激素（TRH）后，血浆浓度在几分钟内升高。