Perez S E, Silva-Netto C R, Saad W A, Camargo L A, Antunes-Rodrigues J
Physiol Behav. 1984 Feb;32(2):191-4. doi: 10.1016/0031-9384(84)90128-8.
Hypotonic and isotonic solutions into the LHA of unrestrained rats caused no alteration in renal water and electrolyte excretion. Similar results were obtained after injecting hypo, iso and hypertonic glucose solutions. On the other hand, a hypertonic NaCl solution produced an increase in natriuresis and kaliuresis immediately after being injected into the LHA. The carbachol elicited an increase in natriuresis and kaliuresis as well as a decrease in urine output in the first urine samples collected after the injection being injected into the LHA. This response was not modified when the same dose of carbachol was injected associated with NaCl or glucose solutions. The observation of a lack of summation between the effects of carbachol and hypertonic NaCl, as well as the maintenance of the natriuretic and kaliuretic effects in response of the injection of the hypertonic solution even in the presence of blocked cholinergic pathways (atropine), suggests a dissociation between the mechanisms activated by the hypertonic solution and the cholinergic stimulation of the LHA.
向未束缚大鼠的下丘脑外侧区注入低渗和等渗溶液，不会改变肾脏的水和电解质排泄。注入低渗、等渗和高渗葡萄糖溶液后也得到了类似结果。另一方面，向下丘脑外侧区注入高渗氯化钠溶液后，立即出现钠利尿和钾利尿增加。将卡巴胆碱注入下丘脑外侧区后，在注射后收集的首批尿液样本中，钠利尿和钾利尿增加，尿量减少。当相同剂量的卡巴胆碱与氯化钠或葡萄糖溶液联合注射时，这种反应没有改变。观察到卡巴胆碱与高渗氯化钠的作用之间缺乏累加效应，以及即使在胆碱能通路被阻断（阿托品）的情况下，注射高渗溶液仍能维持钠利尿和钾利尿效应，这表明高渗溶液激活的机制与下丘脑外侧区的胆碱能刺激之间存在分离。
