对来自草原响尾蛇（绿响尾蛇）毒液的抗肌毒素a抗血清中和肌毒素a及同源粗毒液诱导的局部肌坏死能力的体内试验。

In vivo test of the ability of antiserum to myotoxin a from prairie rattlesnake (Crotalus viridis viridis) venom to neutralize local myonecrosis induced by myotoxin a and homologous crude venom.

作者信息

Ownby C L, Colberg T R, Claypool P L, Odell G V

出版信息

Toxicon. 1984;22(1):99-105. doi: 10.1016/0041-0101(84)90142-9.

PMID:6719480

Abstract

Antiserum to myotoxin a was tested for its ability to prevent local myonecrosis induced by myotoxin a and C. v. viridis venom. Antiserum was injected i.v. either 5 min before or immediately, 15 min, 30 min, 1 hr or 3 hr after i.m. injection of toxin or venom. A light microscopic method was used to measure the effects of myotoxin a, i.e. vacuolation index, and whole venom, i.e. myonecrosis index. The results show that antimyotoxin a serum neutralizes the myotoxicity of a sublethal amount of myotoxin a if injected 56 min before or immediately after toxin, but not if injected 15 min after the toxin. Its neutralizing ability for crude C. v. viridis venom was considerably better, neutralizing a dose of 0.75 microgram/g even if injection of antiserum was delayed for 30 min after venom injection. Thus, antimyotoxin a serum might be useful in treating myonecrosis resulting from prairie rattlesnake (C. v. viridis) venom poisoning.

摘要

检测抗肌毒素a血清预防由肌毒素a和绿睫蝰蛇毒液诱导的局部肌坏死的能力。在肌内注射毒素或毒液前5分钟静脉注射抗血清，或者在注射毒素或毒液后立即、15分钟、30分钟、1小时或3小时静脉注射抗血清。采用光学显微镜方法测量肌毒素a的作用，即空泡化指数，以及全毒液的作用，即肌坏死指数。结果表明，如果在毒素注射前56分钟或注射后立即注射抗肌毒素a血清，可中和亚致死量肌毒素a的肌毒性，但在毒素注射后15分钟注射则不能。其对绿睫蝰蛇粗毒液的中和能力要好得多，即使在毒液注射后30分钟延迟注射抗血清，也能中和0.75微克/克的剂量。因此，抗肌毒素a血清可能有助于治疗草原响尾蛇（绿睫蝰蛇）毒液中毒导致的肌坏死。