采用酶联免疫吸附测定法和免疫扩散法检测三种抗血清中抗肌毒素a和草原响尾蛇（绿拟眼镜蛇）毒液的抗体。
Detection of antibodies to myotoxin a and prairie rattlesnake (Crotalus viridis viridis) venom in three antisera using enzyme-linked immunosorbent assay and immunodiffusion.
作者信息
Ownby C L, Odell G V, Theakston R D
出版信息
Toxicon. 1983;21(6):849-55. doi: 10.1016/0041-0101(83)90074-0.
Immunodiffusion and enzyme-linked immunosorbent assay (ELISA) were used to compare three antisera for their content of antibodies against myotoxin a and C. v. viridis venom. No antibodies were detected in Wyeth's polyvalent (Crotalidae) antivenin against myotoxin a using immunodiffusion, whereas ELISA indicated a low titer of such antibodies. However, antimyotoxin a serum and anti-C. v. viridis venom both had higher titers than antivenin when tested against myotoxin a and crude venom in the ELISA. These results correlate well with previous data which indicated that antiserum to myotoxin a was more effective than antivenin in neutralizing the myotoxicity of C. v. viridis venom. The high content of antibodies to myotoxin a in anti-C. v. viridis venom supports the hypothesis that this monovalent antiserum might be effective in neutralizing rattlesnake (C. v. viridis) venom-induced myonecrosis.
采用免疫扩散法和酶联免疫吸附测定法（ELISA），比较了三种抗血清中抗肌毒素a和竹叶青蛇毒的抗体含量。用免疫扩散法检测发现，惠氏多价（响尾蛇科）抗蛇毒血清中未检测到抗肌毒素a的抗体，而ELISA法显示此类抗体滴度较低。然而，在ELISA法中，当检测抗肌毒素a血清和抗竹叶青蛇毒血清针对肌毒素a和粗毒的反应时，二者的滴度均高于抗蛇毒血清。这些结果与之前的数据高度相关，之前的数据表明，抗肌毒素a血清在中和竹叶青蛇毒的肌毒性方面比抗蛇毒血清更有效。抗竹叶青蛇毒血清中抗肌毒素a抗体含量高，支持了这一假设，即这种单价抗血清可能有效中和响尾蛇（竹叶青）毒液诱导的肌肉坏死。
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