Danforth H D, Chobotar B, Entzeroth R
Z Parasitenkd. 1984;70(2):165-71. doi: 10.1007/BF00942218.
The pathology that occurs in mouse embryonic brain ( MEB ) cells that have been penetrated by sporozoites of Eimeria papillata was studied by light and electron microscopy. At the light microscopy level the greatest number of intracellular parasites was seen at 15 and 45 min postinoculation (PI). The monolayer of MEB cells had begun to round up by 45 min PI, and by 60 min PI most of the cells were stripped from the coverslip. Little ultrastructural damage was seen in MEB cells just penetrated by the parasites at 15 min PI, and no host cell membrane was seen around the sporozoites that had just entered the cells. Flexing and bending of the sporozoites within the MEB cell caused vacuolization of cell cytoplasm and in some cases rupture of host cell membrane. Sporozoites leaving the host cells at 15 min PI caused a rupture of the host cell membrane at the apical end of the parasite, and both host cell membrane and cytoplasm were attached to the surface of the parasite. MEB cells still attached to coverslips at 45 min PI demonstrated complete degeneration.
利用光学显微镜和电子显微镜研究了被乳头艾美耳球虫子孢子侵入的小鼠胚胎脑（MEB）细胞中发生的病理变化。在光学显微镜水平下，接种后（PI）15分钟和45分钟时可见到数量最多的细胞内寄生虫。MEB细胞单层在接种后45分钟开始变圆，到接种后60分钟时，大多数细胞从盖玻片上脱落。在接种后15分钟刚被寄生虫侵入的MEB细胞中几乎未见超微结构损伤，刚进入细胞的子孢子周围未见宿主细胞膜。MEB细胞内子孢子的弯曲和摆动导致细胞质空泡化，在某些情况下宿主细胞膜破裂。接种后15分钟离开宿主细胞的子孢子导致寄生虫顶端的宿主细胞膜破裂，宿主细胞膜和细胞质都附着在寄生虫表面。接种后45分钟仍附着在盖玻片上的MEB细胞呈现完全退化。
