Bauer F, Stachelberger H
Z Lebensm Unters Forsch. 1984;178(2):86-9. doi: 10.1007/BF01088839.
The detection of blood plasma in heat-treated meat products was performed by means of ultrathin-layer isoelectric focusing. Because of the poor solubility of denatured blood plasma in water or diluted salt solutions the extracts were concentrated by adsorption of the protein on hydroxyl apatite and subsequent elution. Desalting of the samples was done by gel chromatography. Gels with a thickness of 150 micron were used for isoelectric focusing because of their excellent resolution as well as low expenditure of time and cost. 0.2% of dry blood plasma in sausages were still detectable by the method described.
采用超薄层等电聚焦法检测热处理肉制品中的血浆。由于变性血浆在水或稀释盐溶液中的溶解度较差，提取物通过蛋白质吸附在羟基磷灰石上并随后洗脱进行浓缩。样品的脱盐通过凝胶色谱法进行。由于具有出色的分辨率以及较低的时间和成本消耗，使用厚度为150微米的凝胶进行等电聚焦。通过所述方法仍可检测到香肠中0.2%的干血浆。
