Yen-Chow Y C, Chow S Y, Woodbury D M
Am J Physiol. 1984 May;246(5 Pt 1):E436-43. doi: 10.1152/ajpendo.1984.246.5.E436.
Sodium distribution in luminal and cellular compartments of turtle thyroid was studied by measurements of radiosodium uptakes into whole thyroid and luminal fluid, sampled with micropipettes, and of Na+ activities in the luminal and cellular fluids of thyroid follicles with a liquid ion-exchange microelectrode. Thyroid water and electrolyte contents were also determined chemically. Radiosodium uptake by both the whole thyroid and by the luminal fluid of control and thyrotropin (TSH)-treated turtles did not reach maximal levels until 16-24h after injection of the radioisotope. In control animals, the volumes of distribution of radiosodium in the whole thyroid and the luminal fluid were much smaller than those calculated from the chemical analysis data for the corresponding tissues. TSH increased the radiosodium spaces to values almost equal to those of the chemically determined sodium spaces. Luminal fluid sodium concentration increased after TSH and decreased after ouabain and furosemide treatments. Intracellular sodium concentration markedly increased after ouabain and decreased after TSH and furosemide. These and our previous results suggest a Na+-I- cotransport mechanism in the thyroid.
通过用微量移液器对整个甲状腺和管腔液进行放射性钠摄取测量，以及用液体离子交换微电极对甲状腺滤泡的管腔液和细胞液中的Na⁺活性进行测量，研究了海龟甲状腺管腔和细胞部分中的钠分布。还通过化学方法测定了甲状腺的水和电解质含量。对照海龟和促甲状腺激素（TSH）处理的海龟的整个甲状腺和管腔液对放射性钠的摄取，直到注射放射性同位素后16 - 24小时才达到最大水平。在对照动物中，整个甲状腺和管腔液中放射性钠的分布体积远小于根据相应组织的化学分析数据计算得出的体积。TSH使放射性钠空间增加到几乎等于化学测定的钠空间的值。TSH处理后管腔液钠浓度升高，哇巴因和呋塞米处理后降低。哇巴因处理后细胞内钠浓度显著升高，TSH和呋塞米处理后降低。这些以及我们之前的结果提示甲状腺中存在Na⁺-I⁻共转运机制。
