Ida S, Tanaka Y, Ohkuma S, Kuriyama K
Anal Biochem. 1984 Feb;136(2):352-6. doi: 10.1016/0003-2697(84)90229-x.
A highly sensitive and specific assay method for cystamine using high-performance liquid chromatography has been developed. The method is based on postcolumn derivatization of cystamine with o-phthaladehyde in the presence of 2-mercaptoethanol and sodium hypochlorite. The separation of cystamine was achieved using a cation exchange column (ISC-05/S0504). The assay was linear over the concentration range of 2 to 200 pmol. For the application of this assay method to biological materials, the pretreatment with a cation exchange column (Dowex 50W X 8) was necessary to remove interfering o-phthaladehyde-reactive substances. Since cysteamine in biological materials was quantitatively converted to cystamine during these sampling procedures, this method was found to be suitable for assaying the cysteamine plus cystamine content in various organs and tissues. The cysteamine-cystamine content in various tissues of rat determined by the present assay method has been presented.
已开发出一种使用高效液相色谱法测定胱胺的高灵敏度和特异性检测方法。该方法基于在2-巯基乙醇和次氯酸钠存在下，胱胺与邻苯二甲醛进行柱后衍生化反应。使用阳离子交换柱（ISC-05/S0504）实现胱胺的分离。该检测在2至200 pmol的浓度范围内呈线性。为了将此检测方法应用于生物材料，需要用阳离子交换柱（Dowex 50W X 8）进行预处理以去除干扰邻苯二甲醛反应性物质。由于生物材料中的半胱胺在这些采样过程中定量转化为胱胺，发现该方法适用于测定各种器官和组织中的半胱胺加胱胺含量。已给出用本检测方法测定的大鼠各种组织中的半胱胺 - 胱胺含量。
