采用火焰光度检测气相色谱法测定半胱胺和胱胺。

Determination of cysteamine and cystamine by gas chromatography with flame photometric detection.

作者信息

Kataoka H, Imamura Y, Tanaka H, Makita M

机构信息

Faculty of Pharmaceutical Sciences, Okayama University, Japan.

出版信息

J Pharm Biomed Anal. 1993 Oct;11(10):963-9. doi: 10.1016/0731-7085(93)80056-7.

DOI:10.1016/0731-7085(93)80056-7

PMID:8305601

Abstract

A gas chromatographic method for the determination of cysteamine and its disulphide cystamine is described. Cysteamine and cystamine are converted into N,S-diisobutoxycarbonyl and N,N-diisobutoxycarbonyl derivatives, respectively. The derivatives are analysed by gas chromatography with flame photometric detection, using a DB-210 capillary column. The calibration curves for cysteamine and cystamine in the range of 0.2-5.0 nmol are linear and sufficiently reproducible for quantitative analysis, and the detection limit is about 0.5 pmol injected. Cysteamine in mouse tissues is found in the free reduced, free oxidized and protein-bound forms. Free oxidized and protein-bound forms are reduced to free cysteamine by the use of sodium borohydride, and then derivatized. Cysteamine and cystamine in mouse tissues can be measured without any interference from coexisting substances by this method. The recoveries of cysteamine and cystamine added to the tissue samples are 91-106%, and their reproducibilities are found to be satisfactory. Analytical results for the determination of various forms of cysteamine in mouse tissues are presented.

摘要

本文描述了一种用于测定半胱胺及其二硫化物胱胺的气相色谱方法。半胱胺和胱胺分别转化为N，S-二异丁氧基羰基和N，N-二异丁氧基羰基衍生物。使用DB-210毛细管柱，通过带有火焰光度检测的气相色谱法对衍生物进行分析。半胱胺和胱胺在0.2 - 5.0 nmol范围内的校准曲线呈线性，且对于定量分析具有足够的重现性，检测限约为进样0.5 pmol。在小鼠组织中，半胱胺以游离还原型、游离氧化型和蛋白结合型存在。通过使用硼氢化钠将游离氧化型和蛋白结合型还原为游离半胱胺，然后进行衍生化。采用该方法可在无共存物质干扰的情况下测定小鼠组织中的半胱胺和胱胺。添加到组织样品中的半胱胺和胱胺的回收率为91 - 106%，且发现其重现性令人满意。文中给出了小鼠组织中各种形式半胱胺测定的分析结果。