使用细菌荧光素酶分析牙菌斑样本中皮摩尔量的L(+)-和D(-)-乳酸。
The analysis of picomole amounts of L(+)- and D(-)-lactic acid in samples of dental plaque using bacterial luciferase.
作者信息
Robrish S A, Curtis M A, Sharer S A, Bowen W H
出版信息
Anal Biochem. 1984 Feb;136(2):503-8. doi: 10.1016/0003-2697(84)90252-5.
L(+)-Lactic acid (5 pmol) and D(-)-lactic acid (20 pmol) were assayed by coupling the generation of NADH with the use of bacterial luciferase. The binding of NADH to L(+)-lactic dehydrogenase made it necessary to denature the protein so that the assay with bacterial luciferase was effective. The coupled luciferase assay of L(+)-lactic acid was 400 times more sensitive than the fluorometric assay. The luciferase coupled assay was used to analyze the L(+)- and D(-)-lactic acid contents of small samples of dental plaque.
通过使用细菌荧光素酶将NADH的生成偶联，对L(+)-乳酸（5皮摩尔）和D(-)-乳酸（20皮摩尔）进行了测定。NADH与L(+)-乳酸脱氢酶的结合使得有必要使蛋白质变性，以便细菌荧光素酶测定有效。L(+)-乳酸的偶联荧光素酶测定比荧光测定法灵敏400倍。荧光素酶偶联测定法用于分析牙菌斑小样本中的L(+)-和D(-)-乳酸含量。
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