Suppr超能文献

利用细菌荧光素酶测定皮摩尔量的还原型烟酰胺腺嘌呤二核苷酸和代谢中间体。

The determination of reduced nicotinamide-adenine dinucleotide and metabolic intermediates in picomole amounts with bacterial luciferase.

作者信息

Golden S, Katz J

出版信息

Biochem J. 1980 Jun 15;188(3):799-805. doi: 10.1042/bj1880799.

DOI:10.1042/bj1880799
PMID:7470036
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1161964/
Abstract

Methods that use bacterial luciferase for the assay of NADH in the range from 1 pmol to 1 nmol are described. Optimal conditions for the assay of glycolytic intermediates, tricarboxylic acid-cycle intermediates and related amino acids from milligram amounts of tissue are presented. The whole spectrum of these intermediates can be determined on about 10 mg of liver tissue. The methods are simple, are suitable for routine use, and the instrumentation is inexpensive. The concentrations of glycolytic intermediates in rat livers were determined by conventional spectrometric methods and with luciferase, and the results found to be in good agreement.

摘要

本文描述了使用细菌荧光素酶测定1皮摩尔至1纳摩尔范围内NADH的方法。介绍了从毫克量组织中测定糖酵解中间产物、三羧酸循环中间产物及相关氨基酸的最佳条件。这些中间产物的全谱可在约10毫克肝脏组织上测定。该方法简单，适用于常规使用，且仪器成本低廉。通过传统光谱法和荧光素酶法测定了大鼠肝脏中糖酵解中间产物的浓度，结果发现两者吻合良好。

相似文献

1
The determination of reduced nicotinamide-adenine dinucleotide and metabolic intermediates in picomole amounts with bacterial luciferase.利用细菌荧光素酶测定皮摩尔量的还原型烟酰胺腺嘌呤二核苷酸和代谢中间体。
Biochem J. 1980 Jun 15;188(3):799-805. doi: 10.1042/bj1880799.
2
The analysis of picomole amounts of L(+)- and D(-)-lactic acid in samples of dental plaque using bacterial luciferase.使用细菌荧光素酶分析牙菌斑样本中皮摩尔量的L(+)-和D(-)-乳酸。
Anal Biochem. 1984 Feb;136(2):503-8. doi: 10.1016/0003-2697(84)90252-5.
3
Immobilization of bacterial luciferase and FMN reductase on glass rods.细菌荧光素酶和FMN还原酶在玻璃棒上的固定化。
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3848-51. doi: 10.1073/pnas.73.11.3848.
4
[Bioluminescent method of determining picomolar amounts of nicotinamide-adenine dinucleotide using an immobilized extract of the luminescent bacterium Beneckea harveyi].[使用哈维贝内克氏菌发光细菌的固定化提取物测定皮摩尔量烟酰胺腺嘌呤二核苷酸的生物发光方法]
Prikl Biokhim Mikrobiol. 1985 Jan-Feb;21(1):114-21.
5
Bioluminescence assay of reduced pyridine and flavine nucleotides with bacterial luciferase.
Arch Int Physiol Biochim. 1971 Jan;79(1):200-1.
6
Simplified luciferase assay of NAD+ applied to microsamples from liver, kidney and pancreatic islets.
Biochim Biophys Acta. 1977 Nov 7;500(1):103-8. doi: 10.1016/0304-4165(77)90050-2.
7
Determination of subpicomole levels of NADH and FMN using bacterial luciferase and the liquid scintillation spectrometer.利用细菌荧光素酶和液体闪烁光谱仪测定亚皮摩尔水平的NADH和FMN。
Anal Biochem. 1971 Feb;39(2):441-53. doi: 10.1016/0003-2697(71)90434-9.
8
Microdetermination of glycerol using bacterial NADH-linked luciferase.
Clin Chim Acta. 1982 Apr 23;120(3):295-300. doi: 10.1016/0009-8981(82)90370-9.
9
Photokinetic micro assay based on dehydrogenase reactions and bacterial luciferase.
Anal Biochem. 1971 Jul;42(1):124-35. doi: 10.1016/0003-2697(71)90018-2.
10
Intermediates in the bacterial luciferase reaction.细菌荧光素酶反应中的中间体。
Biochem Biophys Res Commun. 1973 Jun 19;52(4):1470-4. doi: 10.1016/0006-291x(73)90666-9.

引用本文的文献

1
Rapid changes in oxidative metabolism as a consequence of elicitor treatment of suspension-cultured cells of French bean (Phaseolus vulgaris L.).菜豆（Phaseolus vulgaris L.）悬浮培养细胞经激发子处理后氧化代谢的快速变化。
Plant Mol Biol. 1995 Jan;27(1):59-67. doi: 10.1007/BF00019178.
2
Quantitative analysis of flux along the gluconeogenic, glycolytic and pentose phosphate pathways under reducing conditions in hepatocytes isolated from fed rats.对从喂食大鼠分离出的肝细胞在还原条件下沿糖异生、糖酵解和磷酸戊糖途径的通量进行定量分析。
Biochem J. 1983 Jun 15;212(3):585-98. doi: 10.1042/bj2120585.
3
Quantitative analysis of intermediary metabolism in hepatocytes incubated in the presence and absence of glucagon with a substrate mixture containing glucose, ribose, fructose, alanine and acetate.在有和没有胰高血糖素存在的情况下，用含有葡萄糖、核糖、果糖、丙氨酸和乙酸盐的底物混合物培养肝细胞，对其中间代谢进行定量分析。
Biochem J. 1985 Feb 1;225(3):761-86. doi: 10.1042/bj2250761.

本文引用的文献

1
Enzymic degradation of isotopically labeded compounds. II. Glucose labeled with 14C and tritium.同位素标记化合物的酶促降解。II. 用¹⁴C和氚标记的葡萄糖。
Anal Biochem. 1970 Mar;34:170-9. doi: 10.1016/0003-2697(70)90098-9.
2
Bacterial bioluminescence light emission in the mixed function oxidation of reduced flavin and fatty aldehyde.还原型黄素与脂肪醛混合功能氧化过程中的细菌生物发光。
CRC Crit Rev Biochem. 1978;5(2):163-84. doi: 10.3109/10409237809177143.
3
Simplified luciferase assay of NAD+ applied to microsamples from liver, kidney and pancreatic islets.
Biochim Biophys Acta. 1977 Nov 7;500(1):103-8. doi: 10.1016/0304-4165(77)90050-2.
4
The use of bacterial luciferase and a liquid scintillation spectrometer to assay the enzymatic synthesis of NAD.
Anal Biochem. 1976 Apr;71(2):333-40. doi: 10.1016/s0003-2697(76)80002-4.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验