高等动物晶状体、肝脏和大脑中的眼酸和去甲眼酸。

Ophthalmic and norophthalmic acid in lens, liver, and brain of higher animals.

作者信息

Tsuboi S, Hirota K, Ogata K, Ohmori S

出版信息

Anal Biochem. 1984 Feb;136(2):520-4. doi: 10.1016/0003-2697(84)90255-0.

Abstract

A sensitive high-performance liquid chromatographic method for the determination of nanomole levels of ophthalmic and norophthalmic acid has been described. The procedure is based upon the conversion of amino group to 2,4-dinitrophenyl derivatives and the detection at 420 nm. This method was applied to the determination of the peptides in lens, liver, and brain of several animals. Mean recoveries were 93.6% for ophthalmic acid and 92.3% for norophthalmic acid added to cattle lens homogenate.

摘要

已描述了一种用于测定纳米摩尔水平的眼酸和去甲眼酸的灵敏高效液相色谱法。该方法基于将氨基转化为2,4-二硝基苯基衍生物，并在420nm处进行检测。该方法应用于测定几种动物晶状体、肝脏和大脑中的肽。添加到牛晶状体匀浆中的眼酸平均回收率为93.6%，去甲眼酸平均回收率为92.3%。