生物样品中作为苯并咪唑的甲酸的高效液相色谱测定法。

High-performance liquid chromatographic determination of formate as benzimidazole in biological samples.

作者信息

Ohmori S, Sumii I, Toyonaga Y, Nakata K, Kawase M

机构信息

Faculty of Pharmaceutical Sciences, Okayama University, Japan.

出版信息

J Chromatogr. 1988 Apr 8;426(1):15-24. doi: 10.1016/s0378-4347(00)81922-2.

DOI:10.1016/s0378-4347(00)81922-2

PMID:3384868

Abstract

Formate was determined as benzimidazole by high-performance liquid chromatography after reaction with o-phenylenediamine at 130 degrees C for 2 h in 1 M perchloric acid. The useful concentration range was 1.6-40 mumol/l and the determination limit was 20 pmol. The recoveries from rat liver homogenate and human urine were 90.3 +/- 2.9 and 89.4 +/- 2.5%, respectively. Using this method, the activity of formaldehyde dehydrogenase in biological samples could be measured, and also the formate concentration in the liver and urine of rats to which methanol had been administered.

摘要

在130℃下于1M高氯酸中与邻苯二胺反应2小时后，通过高效液相色谱法将甲酸盐测定为苯并咪唑。有效浓度范围为1.6 - 40μmol/l，测定限为20pmol。大鼠肝匀浆和人尿中的回收率分别为90.3±2.9%和89.4±2.5%。使用该方法，可以测定生物样品中甲醛脱氢酶的活性，以及给予甲醇的大鼠肝脏和尿液中的甲酸盐浓度。