Escudier E, Bernaudin J F, Bernaudin P, Reinert P, Canet J, Boucherat M, Peynegre R
Ann Otolaryngol Chir Cervicofac. 1984;101(2):150-2.
Abnormalities of the ciliary function have been found as an important factor in chronic respiratory diseases. For the study of the ciliary beat frequency, we developed a stroboscopic method using a stroboscopic illuminator ( Strobo Hertz) substituted to the normal light of an inverted optic microscope (Leitz). The ciliated cells obtained either by nasal scraping or tracheobronchial brushing were maintained in a culture medium (IP 199) at 37 degrees C. The ciliary beat frequency was measured at various times, at least one hour after the sampling. Nasal scraping, was performed with a curette in 17 children from 10 months to 16 years who complained of chronic respiratory diseases. Ciliated cells were obtained in 14 samples. In one of them 5 years old with a situs inversus, although the ciliated cells were numerous, the cilia appeared immotile. In the 13 others, the frequency was from 7 to 11.5 Hz with a mean of 10.8 Hz. These results were compared to those obtained by tracheobronchial brushings performed in 7 patients from 4 months to 46 years who underwent an endoscopic investigation. The ciliary frequency was 7 to 10 Hz with a mean of 8.5 Hz. The study of the ciliary beat frequency obtained by nasal scraping seems to be a reliable method for the detection of abnormal ciliary function. Nasal scraping is a non invasive method. Moreover, the stroboscopic method is a non expensive tool.
纤毛功能异常已被发现是慢性呼吸道疾病的一个重要因素。为了研究纤毛摆动频率，我们开发了一种频闪观测法，使用频闪照明器（频闪赫兹）替代倒置光学显微镜（徕卡）的普通光源。通过鼻刮术或气管支气管刷检获得的纤毛细胞在37摄氏度的培养基（IP 199）中培养。在采样后至少一小时的不同时间测量纤毛摆动频率。对17名年龄从10个月至16岁、患有慢性呼吸道疾病的儿童进行鼻刮术，用刮匙采集样本。14个样本中获得了纤毛细胞。其中一名5岁的内脏反位患儿，尽管纤毛细胞数量众多，但纤毛似乎不动。在其他13名患儿中，频率为7至11.5赫兹，平均为10.8赫兹。将这些结果与7名年龄从4个月至46岁、接受内镜检查的患者通过气管支气管刷检获得的结果进行比较。纤毛频率为7至10赫兹，平均为8.5赫兹。通过鼻刮术获得纤毛摆动频率的研究似乎是检测纤毛功能异常的一种可靠方法。鼻刮术是一种非侵入性方法。此外，频闪观测法是一种成本不高的工具。
