Horio S, Kohrogi H, Ando M, Sugimoto M, Araki S
Arch Int Pharmacodyn Ther. 1984 Jan;267(1):80-90.
The effects of nifedipine, a calcium channel blocking agent, on human bronchoconstriction induced by acetylcholine and histamine were studied in vitro. Bronchial preparations were obtained from 13 patients undergoing surgery for bronchial carcinoma. Bronchial strips were mounted for continuous isometric tension recording. Acetylcholine and histamine caused bronchoconstrictions, but the constrictive effects were inhibited by 2.9 X 10(-6) M nifedipine. At a concentration of 2.9 X 10(-6) M nifedipine increased by 22-fold the concentration of acetylcholine required to produce a 50% of maximal contraction of bronchial strips, and by 160-fold the concentration of histamine required. Nifedipine also reversed already established bronchoconstrictions by acetylcholine and histamine. The percentage of contraction at 20 min after 2.9 X 10(-6) M nifedipine was 7 +/- 11.1% (mean +/- SEM) and - 21 +/- 20.0% of the control maximal response by 10(-3) M acetylcholine and 10(-3) M histamine, respectively. Thus, nifedipine clearly prevented and reversed pharmacologically-induced bronchoconstriction in humans. These results suggest that it is valuable to administer nifedipine to prevent and treat asthma attack.
在体外研究了钙通道阻滞剂硝苯地平对乙酰胆碱和组胺诱导的人支气管收缩的影响。从13例接受支气管癌手术的患者获取支气管标本。将支气管条固定用于连续等长张力记录。乙酰胆碱和组胺引起支气管收缩，但2.9×10⁻⁶ M硝苯地平抑制了这种收缩作用。在浓度为2.9×10⁻⁶ M时，硝苯地平使引起支气管条最大收缩50%所需的乙酰胆碱浓度增加了22倍，使所需组胺浓度增加了160倍。硝苯地平还能逆转已由乙酰胆碱和组胺引起的支气管收缩。在2.9×10⁻⁶ M硝苯地平作用20分钟后，10⁻³ M乙酰胆碱和10⁻³ M组胺引起的收缩百分比分别为对照最大反应的7±11.1%（平均值±标准误）和 - 21±20.0%。因此，硝苯地平能明显预防和逆转药理学诱导的人支气管收缩。这些结果表明，给予硝苯地平预防和治疗哮喘发作是有价值的。
