Grant D M, Tang B K, Kalow W
Br J Clin Pharmacol. 1984 Apr;17(4):459-64. doi: 10.1111/j.1365-2125.1984.tb02372.x.
A method is presented for the use of caffeine, in the forms commonly ingested by a large proportion of the world's population, to test for the clinically important acetylation polymorphism. Each of 146 subjects provided a spot sample of urine between 2 and 6 h after coffee, tea or cola soft drink consumption, and the molar ratio of 5-acetylamino-6- formylamino -3-methyluracil ( AFMU ) to 1-methylxanthine (1X) was determined by a simple h.p.l.c. assay. The ratio afforded segregation of three apparent modes of acetylation capacity in this population, in concordance with a standard sulphamethazine phenotyping procedure and with other methods using controlled caffeine intake and urine collections. The day-to-day consistency of the method was established in eight selected subjects.
本文介绍了一种利用咖啡因（世界上很大一部分人口常用的摄入形式）来检测临床上重要的乙酰化多态性的方法。146名受试者在饮用咖啡、茶或可乐软饮料后2至6小时提供一份尿液点样，通过简单的高效液相色谱分析测定5-乙酰氨基-6-甲酰氨基-3-甲基尿嘧啶（AFMU）与1-甲基黄嘌呤（1X）的摩尔比。该比例在这一人群中呈现出三种明显的乙酰化能力模式，与标准的磺胺二甲嘧啶表型分析程序以及其他使用受控咖啡因摄入和尿液收集的方法一致。在八名选定的受试者中确定了该方法的每日一致性。
