Harris R A, Penington D G
Br J Haematol. 1984 May;57(1):37-48.
The administration of low-dose vincristine (VCR) (0.1 mg/kg) to mice resulted in thrombocytosis without prior thrombocytopenia. No significant changes in marrow megakaryocyte numbers were found. However, after a minor early decrease, mean megakaryocyte ploidy increased, with a peak at 3 d. The number of megakaryocyte colony-forming cells (MEG-CFC) in bone marrow did not change significantly. In contrast with the effects on marrow, the concentration of megakaryocytes and the content of MEG-CFC in the spleen were significantly reduced for 1-2 d after VCR. This reduction was followed by a compensatory rise in the splenic content of MEG-CFC (peak 3-fold increase at 3 d), and 1-2 d later, an increase in splenic megakaryocytes which was concurrent with the increased platelet count. Culture of marrow and spleen cells in the presence of VCR resulted in inhibition of megakaryocyte colony formation at concentrations greater than 5 ng/ml and parallel reduction of the number of megakaryocytes per colony and the mean ploidy of colony megakaryocytes. The results suggest that the thrombocytosis induced by low-dose VCR does not result simply from an effect on platelets, but reflects compensatory changes in megakaryopoiesis secondary to toxic suppression of megakaryocytes and their progenitors.
给小鼠注射低剂量长春新碱（VCR）（0.1mg/kg）会导致血小板增多，且无前期血小板减少。未发现骨髓巨核细胞数量有显著变化。然而，在早期轻微下降后，平均巨核细胞倍性增加，在第3天达到峰值。骨髓中巨核细胞集落形成细胞（MEG-CFC）的数量没有显著变化。与对骨髓的影响相反，VCR注射后1-2天，脾脏中巨核细胞的浓度和MEG-CFC的含量显著降低。这种降低之后，脾脏中MEG-CFC的含量出现代偿性升高（第3天峰值增加3倍），1-2天后，脾脏巨核细胞增加，同时血小板计数升高。在VCR存在的情况下培养骨髓和脾细胞，当浓度大于5ng/ml时，会抑制巨核细胞集落形成，并使每个集落中的巨核细胞数量和集落巨核细胞的平均倍性平行降低。结果表明，低剂量VCR诱导的血小板增多并非仅仅源于对血小板的作用，而是反映了巨核细胞及其祖细胞受到毒性抑制后巨核细胞生成的代偿性变化。
