Moskalenko I E, Shtrankfel'd I G
Biofizika. 1984 Mar-Apr;29(2):325-6.
Spectra of tryptophan UV-fluorescence and UV-absorption of myosin isolated 30 days after denervation of white skeletal muscles of rabbit were studied. It has been observed that fluorescence intensity of such myosin in the maximum decreases. This decrease becomes more pronounced after purification of protein from admixtures. It has been shown that after denervation the absorption at 260 nm increases, however, the difference disappears after myosin purification. The data obtained point to a change of myosin structural state after denervation.
研究了兔白色骨骼肌去神经支配30天后分离得到的肌球蛋白的色氨酸紫外荧光光谱和紫外吸收光谱。观察到这种肌球蛋白在荧光强度最大值处降低。从混合物中纯化蛋白质后，这种降低变得更加明显。结果表明，去神经支配后260nm处的吸收增加，然而，肌球蛋白纯化后这种差异消失。所获得的数据表明去神经支配后肌球蛋白结构状态发生了变化。
