Melchior W B, Beland F A
Chem Biol Interact. 1984 Apr;49(1-2):177-87. doi: 10.1016/0009-2797(84)90060-7.
The carcinogens N-acetoxy-2-acetylaminofluorene (N-acetoxy-AAF) and N-hydroxy-2-aminofluorene (N-hydroxy-AF) were incubated with calf thymus DNA to determine if reaction occurred preferentially with discrete regions within the DNA. Derivative melting profiles indicated that both compounds decreased satellite transitions and that N-acetoxy-AAF depressed the melting of higher temperature regions. These data suggest that N-acetoxy-AAF reacted to a greater extent with G + C-rich regions and, because the resulting adduct disrupted the helix, the cooperativity of melting decreased. Reaction of N-acetoxy-AAF with purified satellite III DNA confirmed the preferential interaction of this carcinogen with G + C-rich regions as compared to main component DNA. The derivative melting profile of lambda DNA in the presence of actinomycin D further demonstrated that this type of analysis can detect preferential interactions with specific DNA sequences.
将致癌物N-乙酰氧基-2-乙酰氨基芴（N-乙酰氧基-AAF）和N-羟基-2-氨基芴（N-羟基-AF）与小牛胸腺DNA一起温育，以确定反应是否优先发生于DNA内的离散区域。衍生熔解曲线表明，这两种化合物均降低了卫星峰跃迁，且N-乙酰氧基-AAF降低了较高温度区域的熔解。这些数据表明，N-乙酰氧基-AAF与富含G + C的区域反应程度更大，并且由于形成的加合物破坏了螺旋结构，熔解的协同性降低。N-乙酰氧基-AAF与纯化的卫星III DNA的反应证实，与主要成分DNA相比，该致癌物与富含G + C的区域优先相互作用。在放线菌素D存在下λDNA的衍生熔解曲线进一步证明，这种类型的分析可以检测与特定DNA序列的优先相互作用。
