Charpentier C, Johnstone R A, Lemonnier A, Myara I, Rose M E, Tuli D
Clin Chim Acta. 1984 Apr 27;138(3):299-308. doi: 10.1016/0009-8981(84)90137-2.
Dipeptides in the urine of a patient suffering from dermatological purpura, associated with iminodipeptiduria , were determined by gas chromatography/mass spectrometry. The dipeptides were identified as R-proline and R'-hydroxyproline where R is any one of the residues, glycyl, alanyl, valyl, leucyl, isoleucyl, seryl, aspartyl, glutamyl, prolyl, phenylalanyl and R' is alanyl, valyl, leucyl or isoleucyl, seryl, prolyl, glutamyl, phenylalanyl. The predominance of proline- and hydroxyproline-containing dipeptides and the percentage distributions of the other amino acid residues, R and R', strongly implicate an abnormality of collagen metabolism. Structural assignments are confidently based on (a) gas chromatographic retention times, (b) electron impact mass spectra and automatic comparison with reference to spectra stored in a specialized library, (c) chemical ionization mass spectra with isobutane and methanol as reactant gases and (d) the use of deuteriated acetic anhydride as a derivatizing agent.
采用气相色谱/质谱法测定了一名患有皮肤病性紫癜并伴有亚氨基二肽尿症患者尿液中的二肽。这些二肽被鉴定为R - 脯氨酸和R'- 羟脯氨酸，其中R为甘氨酰、丙氨酰、缬氨酰、亮氨酰、异亮氨酰、丝氨酰、天冬氨酰、谷氨酰、脯氨酰、苯丙氨酰中的任何一种残基，R'为丙氨酰、缬氨酰、亮氨酰、异亮氨酰、丝氨酰、脯氨酰、谷氨酰、苯丙氨酰。含脯氨酸和羟脯氨酸的二肽占优势，以及其他氨基酸残基R和R'的百分比分布，强烈提示胶原蛋白代谢异常。结构归属可靠地基于：(a)气相色谱保留时间；(b)电子轰击质谱，并与存储在专门库中的参考光谱自动比较；(c)以异丁烷和甲醇作为反应气体的化学电离质谱；以及(d)使用氘代乙酸酐作为衍生化试剂。
