Stambolian D, Scarpino-Myers V, Harris H
Exp Eye Res. 1984 Mar;38(3):231-7. doi: 10.1016/0014-4835(84)90161-1.
Thin-layer isoelectric focusing was used to investigate galactokinase in lenses from humans, cows, rats, rabbits and also in other tissues (red cells, liver, kidney, brain, placenta) from these species. In each case the enzyme activity was present as a single isozyme. The isoelectric point was the same in lens, red cell and other tissues of the same species, but differed from species to species. Post-translational modification due to deamidation was not detected in the lens or red cell from these species. Galactokinase activities in whole tissue extract were determined in different tissues of the various species. There were wide variations in activity. In the adult human lens, both normal and cataractous, it was low. The findings indicate that isoelectric focusing of RBC galactokinase combined with specific enzyme staining allows one to monitor lens galactokinase in patients with cataracts.
采用薄层等电聚焦法研究了人类、牛、大鼠、兔子晶状体中的半乳糖激酶，以及这些物种的其他组织（红细胞、肝脏、肾脏、大脑、胎盘）中的半乳糖激酶。在每种情况下，酶活性均以单一同工酶形式存在。同一物种的晶状体、红细胞和其他组织中的等电点相同，但不同物种之间存在差异。在这些物种的晶状体或红细胞中未检测到因脱酰胺作用导致的翻译后修饰。测定了不同物种不同组织中全组织提取物的半乳糖激酶活性。活性存在很大差异。在正常和白内障成人晶状体中，该活性较低。研究结果表明，红细胞半乳糖激酶的等电聚焦结合特异性酶染色可用于监测白内障患者的晶状体半乳糖激酶。
