Brenner S H, Lessing J B, Schoenfeld C, Amelar R D, Dubin L, Weiss G
Fertil Steril. 1984 Jul;42(1):92-6. doi: 10.1016/s0015-0282(16)47964-1.
The effect of relaxin, a peptide hormone present in seminal plasma, on the penetration of cervical mucus by washed human sperm in vitro was studied. Penetration was analyzed by two methods. The total number of sperm per 100 X field at the 10-mm, 20-mm, and 30-mm points along a mucus-filled capillary tube was counted and the farthest distance 100 sperm and 5 sperm/100 X field penetrated the capillary tube was measured. These measurements were made at 30, 60, and 90 minutes. Treating washed sperm with physiologic concentrations of relaxin resulted in significantly increased sperm penetration as compared with sperm treated with albumin or buffer. Replacing seminal plasma to washed sperm resulted in equivalently increased penetration. The findings strongly suggest that relaxin is necessary for proper penetration of cervical mucus by human sperm.
研究了精浆中存在的一种肽类激素松弛素对体外洗涤后的人类精子穿透宫颈黏液的影响。采用两种方法分析穿透情况。沿充满黏液的毛细管在10毫米、20毫米和30毫米处每100倍视野下的精子总数进行计数，并测量100个精子和每100倍视野下5个精子穿透毛细管的最远深度。这些测量在30分钟、60分钟和90分钟时进行。与用白蛋白或缓冲液处理的精子相比，用生理浓度的松弛素处理洗涤后的精子会使精子穿透率显著增加。用精浆替代洗涤后的精子也会使穿透率同等增加。这些发现有力地表明，松弛素是人类精子正常穿透宫颈黏液所必需的。
