Shoham Z, Megory E, Lidor A, Madgar I, Lunenfeld B, Weissenberg R
Institute of Endocrinology, Chaim Sheba Medical Center, Tel-Hashomer, Israel.
Arch Androl. 1987;19(3):261-7. doi: 10.3109/01485018708986826.
The in vitro penetration ability of human sperm into bovine cervical mucus after washing and suspension of the sperm in Earl's medium was compared with that of untreated sperm. The extent of penetration was found to be significantly lower in sperm washed and suspended in Earl's medium than in the unwashed sperm (p less than 0.0001); it increased to some extent on resuspension of the washed sperm in the seminal plasma but still remained significantly lower than that of intact sperm (p less than 0.009). The introduction of caffeine into the Earl's medium resulted in an increased penetration of the washed sperm into bovine cervical mucus, but this was still significantly lower than the penetration of the unwashed sample (p less than 0.02). The data point to the effectiveness of the seminal plasma as a medium facilitating sperm penetration through bovine cervical mucus.
将精子在厄尔氏培养基中洗涤并悬浮后，比较其与人精子未经处理时穿透牛宫颈黏液的体外能力。结果发现，在厄尔氏培养基中洗涤并悬浮的精子的穿透程度显著低于未洗涤的精子（p小于0.0001）；将洗涤后的精子重新悬浮于精浆中后，其穿透程度有所增加，但仍显著低于完整精子（p小于0.009）。在厄尔氏培养基中加入咖啡因后，洗涤后的精子穿透牛宫颈黏液的能力增强，但仍显著低于未洗涤样本（p小于0.02）。这些数据表明精浆作为促进精子穿透牛宫颈黏液的介质具有有效性。
