Exon J H, Koller L D, Henningsen G M, Osborne C A
Fundam Appl Toxicol. 1984 Apr;4(2 Pt 1):278-83. doi: 10.1016/0272-0590(84)90129-5.
A battery of immunoassays were developed for the rat which measured the major arms of the immune system, namely, humoral immunity, cell-mediated immunity (CMI), and macrophage function. Humoral immunity was assessed by the enzyme-linked immunosorbent assay (using keyhole limpet hemocyanin as antigen). CMI was measured by delayed-type hypersensitivity to heat-aggregated bovine serum albumin using a footpad swelling technique. Macrophage function was assessed by measuring the ability of macrophages to phagocytize sheep red blood cells in vitro. The feasibility of performing all three immunoassays in the same animal was investigated by treating groups of rats with all combinations of antigenic exposure used to induce each type of response. Multiple antigenic treatment regimens did not significantly alter responses to individual antigens used in this study. Animals exposed to the concurrent antigen treatment regimens remained sensitive to immunosuppressants. These data suggest that multiple immunoassays can be successfully performed utilizing a single animal which has been concurrently treated with different antigens. The inclusion of additional assays into this protocol and the efficacy to immunotoxicologic testing is discussed.
已为大鼠开发了一系列免疫测定法，用于检测免疫系统的主要分支，即体液免疫、细胞介导免疫（CMI）和巨噬细胞功能。体液免疫通过酶联免疫吸附测定法（使用钥孔戚血蓝蛋白作为抗原）进行评估。CMI通过使用足垫肿胀技术对热聚集牛血清白蛋白的迟发型超敏反应来测量。巨噬细胞功能通过测量巨噬细胞在体外吞噬绵羊红细胞的能力来评估。通过用用于诱导每种类型反应的所有抗原暴露组合处理大鼠组，研究了在同一动物中进行所有三种免疫测定的可行性。多种抗原治疗方案并未显著改变本研究中使用的个体抗原的反应。暴露于同时抗原治疗方案的动物对免疫抑制剂仍保持敏感。这些数据表明，利用同时用不同抗原处理的单个动物可以成功进行多种免疫测定。本文讨论了将额外的测定纳入该方案以及其对免疫毒理学测试的有效性。
