Exon J H, Koller L D
Int J Immunopharmacol. 1983;5(2):131-6. doi: 10.1016/0192-0561(83)90004-8.
Female Sprague - Dawley rats were exposed to 0, 5, 50 or 500 ppm 2-chlorophenol (2-CP) or pentachlorophenol (PCP) from weaning to 3 weeks postparturition after breeding at 90 days of age. Progeny were weaned at 3 weeks of age and continued on chlorophenol treatment for 10 weeks at which time major immune functions were tested. Humoral immunity was measured by an indirect ELISA, cell-mediated immunity was monitored by delayed-type hypersensitivity (DTH) to oxazolone, and macrophage function was tested by phagocytosis of sheep red blood cells. Rats treated with cyclophosphamide were included as a positive immunosuppressed control. PCP-treated rats had significantly decreased antibody titers and DTH response and increased induced peritoneal macrophage numbers which displayed hyperphagocytic activity. Immune responses in rats treated with 2-CP were not significantly different from controls. The data indicate that (1) the immune system may be a sensitive target for PCP toxicity but not for 2-CP, (2) closely related chlorophenolic chemical isomers may exert different toxic effects on the immune system, and (3) PCP can exert depressive effects on some major immune parameters while enhancing others.
将90日龄的雌性斯普拉格-道利大鼠饲养繁殖后，从断奶至产后3周，使其暴露于浓度为0、5、50或500 ppm的2-氯酚（2-CP）或五氯酚（PCP）环境中。子代在3周龄时断奶，并继续接受氯酚处理10周，之后对主要免疫功能进行检测。通过间接酶联免疫吸附测定法（ELISA）测量体液免疫，通过对恶唑酮的迟发型超敏反应（DTH）监测细胞介导免疫，并通过吞噬绵羊红细胞来检测巨噬细胞功能。用环磷酰胺处理的大鼠作为阳性免疫抑制对照。经五氯酚处理的大鼠抗体滴度和DTH反应显著降低，诱导的腹腔巨噬细胞数量增加，表现出高吞噬活性。经2-氯酚处理的大鼠的免疫反应与对照组无显著差异。数据表明：（1）免疫系统可能是五氯酚毒性的敏感靶点，但不是2-氯酚的敏感靶点；（2）结构紧密相关的氯酚化学异构体可能对免疫系统产生不同的毒性作用；（3）五氯酚可对某些主要免疫参数产生抑制作用，同时增强其他参数。
