van Minnen J, Sokolove P G
Gen Comp Endocrinol. 1984 Apr;54(1):114-22. doi: 10.1016/0016-6480(84)90206-5.
Galactogen synthesis in the albumen gland of the slug Limax maximus is under direct endocrine control [J. van Minnen, J. Wijdenes, and P. G. Sokolove (1983) Gen. Comp. Endocrinol. 49, 307-314]. The present results show that dorsal body cells (DBC) present throughout the connective tissue around the circumesophageal brain are the major cellular source of a galactogen synthesis-stimulating factor (GAL-SF). The amount of GAL-SF varies during sexual maturation of L. maximus (a protandrous hermaphrodite): it is low when animals are immature and reaches a peak in the late female phase when the animals prepare to lay eggs. The morphology of DBC also changes during maturation. DBC are small and release little secretory product in immature and early male-phase animals; release is higher and DBC are larger in late female-phase animals. Gel filtration of homogenates of cerebral ganglia on Sephadex G75 yields one peak of GAL-SF activity with an estimated Mr of 4000-7000 Da. Subsequent anion exchange chromatography showed that GAL-SF could be eluted with a linear NaCl gradient; GAL-SF was found in fractions with a NaCl concentration between 0.04 and 0.07 M.
蛞蝓大蛞蝓蛋白腺中的半乳糖原合成受直接内分泌控制[J. 范·明嫩、J. 维耶德内斯和P. G. 索科洛夫（1983年）《普通比较内分泌学》49卷，307 - 314页]。目前的结果表明，环绕食管脑的结缔组织中普遍存在的背体细胞（DBC）是半乳糖原合成刺激因子（GAL - SF）的主要细胞来源。在大蛞蝓（一种先雄后雌的雌雄同体动物）的性成熟过程中，GAL - SF的量会发生变化：在动物未成熟时含量较低，而在动物准备产卵的雌性后期达到峰值。DBC的形态在成熟过程中也会发生变化。在未成熟和雄性早期阶段的动物中，DBC较小且分泌产物释放较少；在雌性后期阶段的动物中，释放量较高且DBC较大。在Sephadex G75上对脑神经节匀浆进行凝胶过滤，得到一个GAL - SF活性峰，估计分子量为4000 - 7000道尔顿。随后的阴离子交换色谱显示，GAL - SF可以用线性NaCl梯度洗脱；在NaCl浓度为0.04至0.07 M的组分中发现了GAL - SF。
