van Minnen J, Wijdenes J, Sokolove P G
Gen Comp Endocrinol. 1983 Feb;49(2):307-14. doi: 10.1016/0016-6480(83)90148-x.
An in vitro method for culturing Limax maximus albumen glands is described in which the biosynthetic activity of the slug albumen gland was monitored by measuring the incorporation of [14C]glucose into galactogen. Homogenates of the central nervous system were shown to cause a 3.5- to 12-fold increase in galactogen synthesis in albumen gland explants as compared to controls. The major sources of the galactogen-synthesis stimulating factor (Gal-SF) were found to be the cerebral ganglia and their surrounding connective tissue. Gal-SF was demonstrated to be peptidase sensitive and heat labile suggesting that it is probably a polypeptide. Autoradiographs of semithin araldite sections supported the incorporation data: in albumen gland explants cultured with cerebral ganglion homogenate considerably more label was found over secretory granules than in control-cultured explants. The possible cellular source of Gal-SF is discussed in relation to its possible origin in other investigated pulmonates.
本文描述了一种培养大蛞蝓蛋白腺的体外方法，其中通过测量[14C]葡萄糖掺入糖原中来监测蛞蝓蛋白腺的生物合成活性。与对照组相比，中枢神经系统匀浆可使蛋白腺外植体中的糖原合成增加3.5至12倍。发现糖原合成刺激因子（Gal-SF）的主要来源是脑神经节及其周围的结缔组织。Gal-SF被证明对肽酶敏感且热不稳定，这表明它可能是一种多肽。半薄环氧树脂切片的放射自显影片支持了掺入数据：在用脑神经节匀浆培养的蛋白腺外植体中，分泌颗粒上发现的标记比对照培养的外植体多得多。结合其在其他已研究肺螺类中的可能来源，讨论了Gal-SF可能的细胞来源。
