Sasaki T, Shintani M, Kihara K
In Vitro. 1984 May;20(5):369-75. doi: 10.1007/BF02619581.
Mechanisms of the inhibition of growth of mammalian cell cultures caused by mycoplasmal infection were investigated by using cell-free extracts of 14 species of mycoplasmas. In four mammalian cell lines tested, the growth of two cell lines, FM3A and MDCK, was inhibited by the extracts of arginine-utilizing mycoplasmas, whereas that of the other two cell lines, Vero and LLC-MK2, was not inhibited by extracts of either arginine- or glucose-utilizing mycoplasmas. These results suggest that there are two types of cell cultures, one susceptible and the other insusceptible to arginine-utilizing mycoplasmas. In a series of experiments using FM3A cells, it was found that the growth inhibition caused by the extracts of arginine-utilizing mycoplasmas was due to removal of arginine from the medium by the action of arginine deiminase present in the extracts and that none of the metabolic products of arginine had any effect on the growth. A highly positive correlation (r = 0.96, P less than 0.01) was observed between the activity of arginine deiminase and the growth-inhibiting activity of extracts of arginine-utilizing mycoplasmas.
利用14种支原体的无细胞提取物，研究了支原体感染导致哺乳动物细胞培养物生长受抑制的机制。在所测试的四种哺乳动物细胞系中，利用精氨酸的支原体提取物抑制了两种细胞系FM3A和MDCK的生长，而利用精氨酸或葡萄糖的支原体提取物均未抑制另外两种细胞系Vero和LLC-MK2的生长。这些结果表明，存在两种类型的细胞培养物，一种对利用精氨酸的支原体敏感，另一种则不敏感。在一系列使用FM3A细胞的实验中，发现利用精氨酸的支原体提取物引起的生长抑制是由于提取物中存在的精氨酸脱亚氨酶作用从培养基中去除了精氨酸，并且精氨酸的任何代谢产物对生长均无影响。在精氨酸脱亚氨酶活性与利用精氨酸的支原体提取物的生长抑制活性之间观察到高度正相关（r = 0.96，P小于0.01）。
