Brade V, Beuscher H U
Immunobiology. 1984 Mar;166(2):177-89. doi: 10.1016/s0171-2985(84)80036-4.
Culture supernatants of thioglycollate-elicited guinea pig peritoneal macrophages contained hemolytic C1, C4, C2 and C3, whereas hemolytic C5, C6, C7, C8 or C9 were not detected. Activity of C1, C2 and C3 increased up to a 48 h culture period, whereas C4 activity already declined in 2 day old cultures. After secretion, the hemolytic activity of C1 was least stable in culture supernatant. Sensitized sheep erythrocytes (EA) when incubated with culture supernatant initiated activation and functional cooperation of secreted C1 to C3 as indicated by formation of EAC142 and EA1423 intermediates. Decay and regeneration with purified C2 was shown for EAC142 and deposition of C3 fragments on EAC1423 was demonstrated with anti-C3. On an average, supernatants of 2 day old macrophage cultures were most suitable for formation of EAC142 and EAC1423 . The rate of EAC142 and EAC1423 formation, and also of C2 and C3 inactivation, during incubation of EA with culture supernatant was slow; addition of purified C1 to culture supernatant, however, greatly enhanced the same reactions of EA with supernatant which indicated that C1 was the rate limiting factor. Local secretion of hemolytic C1, C4, C2 and C3 by macrophages may have an important role in antimicrobial defense mechanisms due to the well-known functional cooperation between macrophages and activated C3.
巯基乙酸盐诱导的豚鼠腹腔巨噬细胞的培养上清液中含有溶血C1、C4、C2和C3，而未检测到溶血C5、C6、C7、C8或C9。C1、C2和C3的活性在培养48小时内升高，而C4活性在培养2天后就已下降。分泌后，C1的溶血活性在培养上清液中最不稳定。致敏绵羊红细胞（EA）与培养上清液孵育时，会引发分泌的C1至C3的激活和功能协同作用，如EAC142和EA1423中间体的形成所示。用纯化的C2对EAC142进行衰变和再生实验，并使用抗C3抗体证明C3片段在EAC1423上的沉积。平均而言，2天龄巨噬细胞培养物的上清液最适合形成EAC142和EAC1423。在EA与培养上清液孵育期间，EAC142和EAC1423的形成速率以及C2和C3的失活速率都很慢；然而，向上清液中添加纯化的C1可大大增强EA与上清液的相同反应，这表明C1是限速因素。由于巨噬细胞与活化的C3之间众所周知的功能协同作用，巨噬细胞局部分泌溶血C1、C4、C2和C3可能在抗菌防御机制中起重要作用。
