Asghar S S, Out J
J Immunol Methods. 1986 Dec 24;95(2):277-81. doi: 10.1016/0022-1759(86)90416-3.
The cellular intermediate EAC14 was successfully prepared by incubating optimally sensitized sheep erythrocytes (EA) with normal serum diluted in complement diluent buffer containing suitable amounts of inhibitors of EAC142 formation from EAC14 and C2, namely polymyxin E and polymyxin B. EAC14 cells could also be prepared by incubating EA with normal serum diluted in Mg2+ free complement diluent buffer in the presence or absence of sucrose. The cellular intermediates prepared by these methods exhibited characteristic EAC142 formation and decay profiles and were found to be suitable for C2 estimations.
通过将最佳致敏绵羊红细胞（EA）与在含有适量EAC14和C2形成抑制剂（即多粘菌素E和多粘菌素B）的补体稀释缓冲液中稀释的正常血清孵育，成功制备了细胞中间体EAC14。EAC14细胞也可以通过在有或没有蔗糖存在的情况下，将EA与在无Mg2+补体稀释缓冲液中稀释的正常血清孵育来制备。通过这些方法制备的细胞中间体表现出特征性的EAC142形成和衰变曲线，并且发现适用于C2的估计。
